جهت دسترسی به کاربرگه ی زیر، از این لینک استفاده کنید. http://192.168.1.35:80/jspui/handle/1842/645
Title: Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells
Authors: Niwa, Hitoshi;Masui, Shinji;Chambers, Ian;Smith, Austin G;Miyazaki, Jun-ichi
Keywords: Phenotypic Complementation
Embryonic
Stem Cells
gene
Issue Date: 6-Jan-2005
Publisher: Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Description: Transcription factors of the POU family govern cell fate through combinatorial interactions with coactivators and corepressors. The POU factor Oct-3/4 can define differentiation, dedifferentation, or self-renewal of pluripotent embryonic stem (ES) cells in a sensitive, dose-dependent manner (H. Niwa, J.-I. Miyazali, and A. G. Smith, Nat. Genet. 24:372–376, 2000). Here we have developed a complementation assay based on the ability of Oct-3/4 transgenes to rescue self-renewal in conditionally null ES cells and used this to define which domains of Oct-3/4 are required to sustain the undifferentiated stem cell phenotype. Surprisingly, we found that molecules lacking either the N-terminal or C-terminal transactivation domain, though not both, can effectively replace full-length Oct-3/4. Furthermore, a fusion of the heterologous transactivation domain of Oct-2 to the Oct-3/4 POU domain can also sustain self-renewal. Thus, the unique function of Oct-3/4 in ES cell propagation resides in combination of the specific POU domain with a generic proline-rich transactivation domain. Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation.
URI: http://192.168.1.35:80/jspui/handle/1842/645
Other Identifiers: MOLECULAR AND CELLULAR BIOLOGY, Mar. 2002, p. 1526–1536
DOI: 10.1128/MCB.22.5.1526–1536.2002
http://hdl.handle.net/1842/645
Type Of Material: Article
Appears in Collections:School of Biological Sciences

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